Bone alkaline phosphatase kinetics studied by a new method
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 50 (3) , 251-259
- https://doi.org/10.1007/bf00491072
Abstract
Studies of enzyme kinetics by histochemical methods has been seriously impaired by available methods. This study combines two newer methods, i.e., sectioning undecalcified bone from glycol methacrylate and the use of atomic absorption spectrophotometry for photographic densitometry. The results indicate that the substrate concentration for zero order kinetics of the alkaline phosphatase reaction in bone is 0.5 mg/ml of substrate; and the Km of the same reaction is estimated to be 0.25 mg/ml or 0.56 mM of substrate.Keywords
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