The Preparation and Characterization of an Aggregated Gliadin Fraction from Wheat

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Abstract
An aggregated gliadin fraction was prepared by gel filtration on Sephacryl S300 in a solvent containing 6.0 M guanidine hydrochloride. This was reduced, alkylated, and separated by ion exchange chromatography. SDS-PAGE of the resulting fraction showed a number of polypeptides, mostly with apparent M1s of around 44 000. The amino acid composition was similar to those reported previously for monomeric α-, β- and γ-gliadins. Automated amino acid sequencing from the N-terminus also showed the presence of sequence types characteristic of α-, β-, and γ-gliadins, but the major sequence type was not related to any described previously. This sequence was NH2-Ser-His-Ile-Pro-Gly-Leu-Glu-Arg-Pro-Ser-Gln-Gln-Gln-Gln-Leu-.