A sensitive spectrophotometric method for lipid hydroperoxide determination

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Abstract
A spectrophotometric method for the quantitation of lipid hydroperoxides is described. The chromophore produced in the reaction is the strongly absorbing I3−ion, which has been used previously by many researchers. The present method deviates from earlier methods in three respects—first, the assay is done at low acid concentrations; second, the iodide concentration is reduced; and third, Fe2+is used as a catalyst. As a result of these modifications the interference from oxygen is minimized. The assay is fast, sensitive and simple to perform.