False-Positive Reactions in the Immunoprecipitation Test for Meat Identification

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Abstract
During the course of experiments into the specificity of the ring precipitation test it was found that a commercial anti-porcine serum reacted with bovine plasma. When this phenomenon was investigated it was found to be non-immunological in nature and that plasma obtained from blood treated with calcium chelating agents gave a positive reaction with normal serum. The species of origin of the reactants appeared to have no effect on the reaction. Further analysis indicated that a similar false-positive reaction could be obtained using sodium ascorbate either as an anticoagulant or as an additive to ground beef. Preliminary studies were conducted on the mechanism of this reaction.

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