Demonstration of protease isoenzymes by two-dimensional polyacrylamide electrophoresis
- 1 January 1983
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 79 (3) , 483-486
- https://doi.org/10.1007/bf00491782
Abstract
Summary A two-dimensional polyacrylamide electrophoresis system is described for the detection of protease isoenzymes using 4-methoxy-2-naphthylamine derivates as substrates. With this technique it is possible to detect isoenzymes differing in molecular weight by separation according to size and shape. This is not possible by the use of isoelectric focusing alone.Keywords
This publication has 7 references indexed in Scilit:
- Investigation of proteases with chromogenic substrates after isoelectric focusing (IEF)Histochemistry and Cell Biology, 1984
- Isoelectric focusing (IEF) and band detection with fluorogenic protease substratesHistochemistry and Cell Biology, 1984
- Contributions of Histochemistry to the Development of the Proteolytic Enzyme Detection System in Diagnostic MedicineJournal of Histochemistry & Cytochemistry, 1983
- The usefulness of the analytical electrofocusing in a thin-layer polyacrylamide gel (PAG) in the histochemistry of enzymes cleaving peptide bondsHistochemistry and Cell Biology, 1981
- Enzyme HistochemistryPublished by Springer Nature ,1979
- Preparation of delipidized serum protein for use in cell culture systemsIn Vitro Cellular & Developmental Biology - Plant, 1976
- High resolution two-dimensional electrophoresis of proteins.Journal of Biological Chemistry, 1975