A new approach to the characterization of steroids containing the 17-α-ketol side chain

Publisher Website
Google Scholar
Abstract
A procedure is described for the characterization of the steroid 17[beta]-ketol side chain by comparison of the mobilities of the steroid, its 21-dehydro and etienic acid derivatives by using thin-layer chromatography. A technique for preparing these derivatives on a small scale is described. The difference in RM, i.e. [DELTA]Rm between the 21-hydroxy steroids and 21-dehydro steroids was constant for 13 steroids; [DELTA]RM values for etienic acids were divided into two groups, dependent on the presence or absence of a 17[alpha]-hydroxy group. By using the procedure described, steroids that contained the 17-ketol side chain could be readily distinguished from those that did not contain this grouping.