Primary structure of the neutral carbohydrate chains of hemocyanin from Panulirus interruptus
- 1 September 1986
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 159 (2) , 297-301
- https://doi.org/10.1111/j.1432-1033.1986.tb09867.x
Abstract
The N-glycosidic carbohydrate chains of Panulirus interruptus hemocyanin, consisting of only mannose and N-acetylglucosamine residues, were liberated by hydrazinolysis of a pronase digest of the purified glycoprotein. The carbohydrate chains were fractionated by high-voltage paper electrophoresis, yielding mainly neutral oligosaccharide-alditols. These were further separated by high-performance liquid chromatography and characterized by 500-MHz 1H-NMR spectroscopy as (formula; see text).Keywords
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