Effect of Warming Rates on the Viability of Frozen Fungous Spores

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Abstract
A study was made of the effect of warming rates on the recovery of fungous spores frozen and stored at liquid-N temperature. Recoveries approximating those of the prefreesse controls were obtained with representative strains of all the 10 sp. studied following rapid and slow cooling when the spores were warmed rapidly. Slower warming rates resulted in marked injury to spores of some spp., especially when cooling was rapid. Of 10 fungi studied, only Cephallophora irregularis sustained a substantial loss of viable cells when warmed at the slow rate following both rapid and slow cooling. The results suggest that most fungi would tolerate rapid or slow freezing if warming was rapid.