Analysis ofDrosophila proboscipediamutant alleles
- 1 June 2004
- journal article
- Published by Canadian Science Publishing in Genome
- Vol. 47 (3) , 600-609
- https://doi.org/10.1139/g03-133
Abstract
Proboscipedia (PB) is a HOX protein required for adult maxillary palp and proboscis formation. To identify domains of PB important for function, 21 pb point mutant alleles were sequenced. Twelve pb alleles had DNA sequence changes that encode an altered PB protein product. The DNA sequence changes of these 12 alleles fell into 2 categories: missense alleles that effect the PB homeodomain (HD), and nonsense or frameshift alleles that result in C-terminal truncations of the PB protein. The phenotypic analysis of the pb homeobox missense alleles suggests that the PB HD is required for maxillary palp and proboscis development and pb – Sex combs reduced (Scr) genetic interaction. The phenotypic analysis of the pb nonsense or frameshift alleles suggests that the C-terminus is an important region required for maxillary palp and proboscis development and pb–Scr genetic interaction. PB and SCR do not interact directly with one another in a co-immunoprecipitation assay and in a yeast two-hybrid analysis, which suggests the pb–Scr genetic interaction is not mediated by a direct interaction between PB and SCR.Key words: proboscipedia, Sex combs reduced, Hox genes, mutant analysis, Drosophila body plan, appendage development.Keywords
This publication has 16 references indexed in Scilit:
- Proboscipedia represses distal signaling in the embryonic gnathal limb fields of Tribolium castaneumWilhelm Roux' Archiv für Entwicklungsmechanik der Organismen, 2003
- Missense mutations of human homeoboxes: A reviewHuman Mutation, 2001
- Comparison of X-ray and NMR structures for the Antennapedia homeodomain–DNA complexNature Structural & Molecular Biology, 1998
- A homeodomain point mutation of the Drosophila proboscipedia protein provokes eye loss independently of homeotic functionMechanisms of Development, 1997
- Dampening of Bait Proteins in the Two-Hybrid SystemAnalytical Biochemistry, 1997
- Homeotic transformation of legs to mouthparts by proboscipedia expression in Drosophila imaginal discsMechanisms of Development, 1997
- Direct regulation of decapentaplegic by Ultrabithorax and its role in Drosophila midgut morphogenesisCell, 1994
- A novel genetic system to detect protein–protein interactionsNature, 1989
- Expression of the Sex combs reduced protein in Drosophila larvaeDevelopmental Biology, 1988
- Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteinsNature, 1984