Influence of Glucose & Light on Pyruvate Metabolism by Starved Cells of Chlorella elipsoida

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Abstract
Glucose increases the incorporation of pyruvate into the ninhydrin-positive fraction of the acidhydrolysate ("proteins"), and light the incorporation into both "proteins" and fats. The interaction of succinate with pyruvate or glucose - or pyruvate-induced respiration by fluoroacetate indicate that the tricarboxylic acid cycle is the major pathway for glucose or pyruvate oxidation. The incorporation of pyruvate into the "proteins" or fats appears, to be limited by the supply of reduced TPN. The metabolism of acetate is further curtailed by the rate of synthesis of dicarboxylic acids. The efficiency with which the energy produced by the oxidation of a substrate is coupled to synthetic reactions (C O2/ algae C14) appears to be dependent on a balanced supply of carbon skeletons, energy, and specific pyridine nucleotides.