Carbon-13 NMR of the bases of three DNA oligonucleotide duplexes: assignment methods and structural features
- 4 October 1988
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 27 (20) , 7902-7909
- https://doi.org/10.1021/bi00420a048
Abstract
Natural abundance 13C NMR spectra of three DNA oligomers have been obtained. Most of the base resonances are well resolved from one another. A combination of two independent methods was used in making assignments: a one-dimensional spectral comparison method and a two-dimensional proton-detected 1H-13C correlated experiment for the protonated carbons. There are large shielding changes (between 1.62 and -1.40 ppm) upon thermal dissociation of the duplex. The shapes of the chemical shift vs temperature curves are largely independent of sequence. The base carbon resonance frequencies are sensitive to hydrogen bonding, base stacking, sugar conformation, and changes in the glycosyl torsion angle.This publication has 26 references indexed in Scilit:
- Conformational microheterogeniety in a DNA double helix: Structure of restriction endonuclease Bam H1 recognition siteBiochemical and Biophysical Research Communications, 1985
- Ab-initio Quantum Mechanical Calculations of NMR Chemical Shifts in Nucleic Acid Constituents I The Watson-Crick Base PairsJournal of Biomolecular Structure and Dynamics, 1984
- Conformation and Dynamics of Short DNA Duplexes: (dC-dG)3and (dC-dG)4Journal of Biomolecular Structure and Dynamics, 1984
- Kinked DNA in crystalline complex with EcoRI endonucleaseNature, 1984
- Multiple quantum two-dimensional 1H--15N nuclear magnetic resonance spectroscopy: chemical shift correlation maps for exchangeable imino protons of Escherichia coli tRNAMetf in water.Proceedings of the National Academy of Sciences, 1983
- Sequence-dependent conformation of an A-DNA double helixJournal of Molecular Biology, 1983
- Carbon 13 spin-lattice relaxation, linewidth, and nuclear Overhauser enhancement measurements of nucleosome length DNA.Journal of Biological Chemistry, 1981
- Structure of a B-DNA dodecamer: conformation and dynamics.Proceedings of the National Academy of Sciences, 1981
- Detection of proton acceptor sites of hydrogen bonding between nucleic acid bases by the use of carbon-13 magnetic resonanceJournal of the American Chemical Society, 1977
- On the atomic or “local” contributions to proton chemical shifts due to the anisotropy of the diamagnetic susceptibility of the nucleic acid basesBiochemical and Biophysical Research Communications, 1976