Multiple quantum two-dimensional 1H--15N nuclear magnetic resonance spectroscopy: chemical shift correlation maps for exchangeable imino protons of Escherichia coli tRNAMetf in water.

Abstract
A procedure based on multiple quantum 2 dimensional NMR spectroscopy is described for generation of 1H-15N chemical shift correlation maps. The method is used to obtain 15N chemical shifts for the exchangeable imino protons in 1H-15N units of site-specifically labeled E. coli tRNAfMet in water. The high sensitivity and excellent chemical shift dispersion of the multiple quantum 2-dimensional technique make it ideally suited for studying protonated N by NMR.

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