Synthesis of Oligonucleotides Containing the Abasic Site Model Compound 1,4-Anhydro-2-Deoxy-D-Ribitol

Abstract
An improved approach for the synthesis of phosphotriester and phosphoramidite derivatives of the 1,4-anhydro-2-deoxy-D-ribitol is presented. The incorporation of these compounds in synthetic DNA and the insertion of purine deoxyribonucleotides opposite the reduced abasic site by DNA polymerase is described.