COMPARISON OF THE DIRECT ANTIGLOBULIN ROSETTING REACTION (DARR) AND DIRECT IMMUNOFLUORESCENCE (DIF) FOR DEMONSTRATION OF SLG-BEARING LYMPHOCYTES IN PIGS, SHEEP AND CATTLE

  • 1 January 1979
    • journal article
    • research article
    • Vol. 36  (3) , 549-555
Abstract
Tests with untreated and trypsin-treated red cells (RBC) from a variety of species showed that anti-Ig[immunoglobulin]-coupled pig RBC are good indicator cells for the study of ruminant blood s[surface]Ig+ lymphocytes by the DARR test. Coupled donkey and rabbit RBC were suitable for investigating pig lymphocytes. The different species showed the following percentages of sIg+ lymphocytes (mean .+-. SE) by DIF and the DARR, respectively: pigs 9.2 .+-. 0.7% and 16.3 .+-. 1.2%; sheep 20.1 .+-. 1.2% and 33.1 .+-. 1.6%; cattle 13.5 .+-. 1.4% and 28.9 .+-. 3.5%. The mean ratio of sIg+ lymphocytes shown by the 2 tests (DARR/DIF) for each species was 1.80 .+-. 0.08 for pigs, 1.73 .+-. 0.7 for sheep and 2.15 .+-. 0.18 for cattle. Preincubation of pig and sheep lymphocytes at 37.degree. C for 1 h did not alter the proportion of sIg+ lymphocytes detected by either test. Thus the DARR test revealed a further population of sIg+ lymphocytes in addition to that detected by immunofluorescence, whose number is proportional to the B [bone marrow-derived] population as measured by DIF and whose sIg is intimately associated with the membrane.