Linked transport of phosphate, potassium ions and protons in Escherichia coli

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Abstract
Pi entry into E. coli cells through either of the 2 Pi-transport systems (Pit or Pst) prompts the influx of K+ and H+ in a ratio that depends on the external pH. The entry of Pi is absolutely dependent on the presence of K+, and the entry of K+ is equally dependent on the presence of Pi. Experiments with a number of mutants carrying any 1 functional Pi-transport system and 1 or more of the individual K+-transport systems indicate a permissive type of linkage of the 2 transports, in that there is no obvious preference by any of the Pi-transport systems for a particular K+-transport system for the concomitant entry of the 2 ions.