Some effects of ouabain and potassium on transport and metabolism in rat small intestine

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Abstract
Ouabain (10-3 [image]) inhibited the transfer of glucose, proline, methionine and fluid but not of galactose and 3-O-methyl glucose by sacs of rat everted intestine. Ouabain (10~3 [image]) inhibited both glucose and mannose metabolism. Hexose metabolism was also reduced by varying the [K] from 4.8 m-equiv/1. Ouabain (10-3 [image]) or absence of K reduced the stimulation of amino acid, hexose and fluid transfer by mannose. Although presence of ouabain or absence of K did not inhibit endogenous galactose transfer, these conditions prevented mannose from stimulating this transfer. Ouabain (10-3 [image]) exerted greater effects on hexose and amino acid transfer when K was omitted from the saline and its inhibitory effects were partially overcome by increased serosal [K]. Ouabain (10-2 [image]) caused further inhibition of amino acid and glucose transfer and also reduced the transfer of galactose and 3-O-methyl glucose. The implications of these results are discussed with respect to the relationships between cations and hexose and amino acid transfer.