INCIDENCE OF ACTIVATING RAS ONCOGENE MUTATIONS ASSOCIATED WITH PRIMARY AND METASTATIC HUMAN-BREAST CANCER
- 15 January 1989
- journal article
- research article
- Vol. 49 (2) , 357-360
Abstract
To test the hypothesis that ras activation is involved in the final stages of breast cancer progression, we analyzed tumor DNA derived from 60 different patients and extracted from 40 invasive primary breast tumors, seven lymph node and skin metastases, nine metastatic effusions, and five established breast cancer cell lines. The polymerase chain reaction technique was used to amplify DNA fragments containing Kirsten-(Ki-), Harvey-(Ha-), and N-ras condons 12, 13, and 61 which were then probed on slot-blots with labeled synthetic oligomers to detect nonconservative single-base mutations. Activating mutations were found in one of 40 primary tumors (Ki-ras condon 13), zero of seven lymph node and skin metastases, one of nine metastatic effusions (Ki-ras condon 12), and two of five cell lines (Ki-ras condons 12 and 13). These results indicate that activating ras mutations are rarely involved in either the initiation or metastatic progression of human breast cancer.This publication has 17 references indexed in Scilit:
- ras GENESAnnual Review of Biochemistry, 1987
- Immortalization in Culture: Occurrence at a Late Stage in the Progression of Breast Cancer2JNCI Journal of the National Cancer Institute, 1987
- MUTATIONS IN N-RAS PREDOMINATE IN ACUTE MYELOID-LEUKEMIA1987
- Purification of DNA from formaldehyde fixed and paraffin embedded human tissueBiochemical and Biophysical Research Communications, 1985
- Amino-acid substitutions at codon 13 of the N-ras oncogene in human acute myeloid leukaemiaNature, 1985
- Transfection of v- ras H DNA into MCF-7 Human Breast Cancer Cells Bypasses Dependence on Estrogen for TumorigenicityScience, 1985
- A position 12-activated H-ras oncogene in all HS578T mammary carcinosarcoma cells but not normal mammary cells of the same patient.Proceedings of the National Academy of Sciences, 1984
- Mechanism of activation of an N-ras gene in the human fibrosarcoma cell line HT1080.The EMBO Journal, 1984
- Activation of Ki-ras2 gene in human colon and lung carcinomas by two different point mutationsNature, 1983
- Mechanism of activation of a human oncogeneNature, 1982