Cryopreservation of Platelets Simplified: A Modified Glycerol‐Glucose Method
- 8 July 1980
- journal article
- research article
- Published by Wiley in Transfusion
- Vol. 20 (4) , 427-432
- https://doi.org/10.1046/j.1537-2995.1980.20480260274.x
Abstract
Human platelets were prepared for freezing in liquid N by prediluting with 1 ml glycerol-glucose freezing solution, allowing the platelet concentrate to set for 10 min at 29.degree. C, and then adding an additional 8 ml at glycerol-glucose freezing solution. A 3-mm thick cardboard holder was constructed and used to hold the platelet concentration during immersion and storage in liquid N. The mean recovery of frozen thawed platelets was 79%. EM examination of the frozen thawed platelets showed the platelets to be structurally intact. In vitro testing showed the platelets to be functional.This publication has 21 references indexed in Scilit:
- A low-viscosity epoxy resin embedding medium for electron microscopyPublished by Elsevier ,2004
- Platelet PreservationTransfusion, 1978
- CRYOPRESERVING HLA-TYPED PLATELETS OBTAINED ON CELL SEPARATORSThe Lancet, 1977
- Platelet preservation V. Survival, serotonin uptake velocity, and response to hypotonic stress of fresh and cryopreserved human plateletsTransfusion, 1977
- Rapid chemical dehydration of samples for electron microscopic examinations.Journal of Histochemistry & Cytochemistry, 1975
- The Platelet Response to Hypotonic Shock. Its Value as an Indicator of Platelet Viability After StorageTransfusion, 1974
- Platelet Preservation by Freezing. Use of Dimethylsulfoxide as Cryoprotective AgentTransfusion, 1974
- Dimethylacetamide, a New Cryoprotective Agent for PlateletsTransfusion, 1971
- Preparation and in Vivo Circulation of Human Platelets Preserved with Combined Dimethylsulfoxide and DextroseTransfusion, 1966
- Toxicological studies on synthetic glycerinJournal of the American Pharmaceutical Association (Scientific ed.), 1950