Effects of metal ions, including Mg2+ and lanthanides, on the cleavage of ribonucleotides and RNA model compounds.
- 15 May 1991
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 88 (10) , 4080-4083
- https://doi.org/10.1073/pnas.88.10.4080
Abstract
The cyclization/cleavage of 3',5'-uridyluridine to form 2',3'-cyclic uridylic acid is very effectively catalyzed by Eu3+, and the cyclization/cleavage of the 1-p-nitrophenyl phosphate ester of propane-1,2-diol also shows strong metal ion catalysis by Eu3+, Tb3+, and Yb3+. It also shows moderate catalysis by Mg2+, but not by Ca2+; Zn2+ and Pb2+ are also good catalysts. Various ligands activate these reactions further, and imidazole apparently acts as an additional base catalyst. Some cyclodextrin derivatives act to bind both the substrate and the metal ion but, contrary to what is reported elsewhere, there is no strong selectivity among nucleotides that can be ascribed to cyclodextrin binding.Keywords
This publication has 10 references indexed in Scilit:
- Visualizing the Higher Order Folding of a Catalytic RNA MoleculeScience, 1991
- Probing the environment of lanthanide binding sites in yeast tRNAPhe by specific metal‐ion‐promoted cleavagesEuropean Journal of Biochemistry, 1989
- On the mechanism of action of ribonucleases: dinucleotide cleavage catalyzed by imidazole and Zn2+.Proceedings of the National Academy of Sciences, 1989
- Identification of the magnesium, europium and lead binding sites in E. coli and lupine tRNAPhe by specific metal ion‐induced cleavagesFEBS Letters, 1989
- Ribonuclease P: An Enzyme with a Catalytic RNA SubunitPublished by Wiley ,1989
- Crystallographic and biochemical investigation of the lead(II)-catalyzed hydrolysis of yeast phenylalanine tRNABiochemistry, 1985
- Pb(II)-catalysed cleavage of the sugar–phosphate backbone of yeast tRNAPhe—implications for lead toxicity and self-splicing RNANature, 1983
- The interaction of metal ions with polynucleotides and related compounds. 13. The effect of metal ions on the enzymatic degradation of ribonucleic acid by bovine pancreatic ribonuclease and of deoxyribonucleic acid by bovine pancreatic deoxyribonuclease I.1969
- Interactions of metal ions with polynucleotides and related compounds. III. Degradation of polyribonucleotides by lanthanum ions,Biopolymers, 1965
- *VERHALTEN UND SPEZIFITAT VON CER UND LANTHAN ALS PHOSPHATASE-MODELLE GEGENUBER NUCLEINSAUREN UND MONONUCLEOTIDEN1954