Synthesis of nuclear proteins during DNA repair synthesis in human diploid fibroblasts damaged with ultraviolet radiation of N-acetoxy-2-acetylaminofluroene.

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Abstract
The accumulation of newly synthesized nuclear proteins in nuclei was examined during DNA repair synthesis in confluent WI-38 human diploid [embryo lung] fibroblasts damaged with UV radiation or N-acetoxy-2-acetylaminofluorene. In contrast to a marked stimulation of DNA repair synthesis, stimulation of amino acid incorporation into histone polypeptides or the various MW classes of nonhistone nuclear proteins was not observed. These results suggest that detectable stimulation of newly synthesized nuclear protein incorporation into nuclei does not accompany DNA repair synthesis induced by UV radiation or a direct acting chemical carcinogen. At least for the special case of repair, DNA synthesis may be uncoupled from histone synthesis.