Synthese der [LysA13] Rinderinsulin-A-Kette in der Form [Lys(Tfa)A13]A(SO3H)4undNαA1-Msc-[LysA13]A(SO3H)4unter Verwendung des S-tert-Butylmercapto-Restes als Thiolschutzgruppe

Abstract
Although the intermediate S-tert-butylmercapto-cysteinyl-peptide derivatives showed a good solubility in organic solvents, the resulting fully protected A chain derivatives had a poor solubility in organic solvents and therefore were deblocked converted into the tetra(S-sulfonic acid) derivatives and purified via ion exchange chromatography.