Removal of background label in immunocytochemistry with the apolar lowicryls by using washed protein A–gold‐precoupled antibodies in a one‐step procedure
- 4 February 1989
- journal article
- research article
- Published by Wiley in Journal of Electron Microscopy Technique
- Vol. 11 (2) , 109-116
- https://doi.org/10.1002/jemt.1060110204
Abstract
Antiserum mixed with protein A–gold produces aggregates that can be washed by centrifugation and redissociated by ultrasonication. These precoupled antibodies are used for immunocytochemical labeling in a one‐step procedure and reveal a very low background on sections of material embedded in polar and apolar Lowicryls. Controls have shown that the background label stems from serum components with only one protein A binding site and is discarded during the wash steps of the precoupling procedure.Keywords
This publication has 18 references indexed in Scilit:
- Pitfalls of immunogold labeling: analysis by light microscopy, transmission electron microscopy, and photoelectron microscopy.Journal of Histochemistry & Cytochemistry, 1987
- Transverse sectioning of plastic-embedded immunolabeled cryosections: Morphology and permeability to protein A-colloidal gold complexesJournal of Ultrastructure and Molecular Structure Research, 1986
- Post‐embedding cytochemistry with gold‐labelled reagents: a reviewJournal of Microscopy, 1986
- Developments of new Lowicryl® resins for embedding biological specimens at even lower temperaturesJournal of Microscopy, 1986
- Soil-borne wheat mosaic virus protein interactions with wheat streak mosaic virus cylindrical inclusionsJournal of Ultrastructure and Molecular Structure Research, 1986
- Chronology of appearance of barley stripe mosaic virus protein in infected wheat cellsJournal of Ultrastructure Research, 1984
- Immunolabeling of adenohypophysial cells with protein A-colloidal gold--antibody complex for electron microscopy: use of the freeze-substitution technique in tissue preparation.Journal of Histochemistry & Cytochemistry, 1984
- Dehydration and embedding temperatures affect the antigenic specificity of tubulin and immunolabeling by the protein A-colloidal gold technique.Journal of Histochemistry & Cytochemistry, 1983
- Immunohistochemical localization of barley stripe mosaic virions in infected wheat cellsJournal of Ultrastructure Research, 1983
- The preparation of protein A-gold complexes with 3 nm and 15 nm gold particles and their use in labelling multiple antigens on ultra-thin sectionsJournal of Molecular Histology, 1982