Oligonudeotides complementary to a promoter over the region −8… + 2 as transcription primers forE. coliRNA polymerase
Open Access
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (22) , 8509-8524
- https://doi.org/10.1093/nar/12.22.8509
Abstract
Primer-dependent transcription by E. coli RNA polymerase on T7 promoter A2 has been studied. Synthetic deoxyribonucleotides complementary to the promoter over the region −8…+2 were taken as primers. A ribonucleoside residue was present at the 3′-end of some of these oligonucleotides. The octanucleotide complementary to the region −8…−1 appeared to be an active primer. Oligonucleotides having lengths from 3 to 6 nucleotide residues complementary to the promoter over the region −4…+2 also exhibited primer activity. The latter was some 5–10 times greater in the case of oligonuoleotides having a ribonucleoside residue at the 3′-end. Oligonucleotides which on complementary binding do not reach the center of phoaphodiester bond synthesis, as well as the decanucleotides (−8…+2) and octanucleotides (−6…+2) of both the ribo- and deoxyribo-series were inactive as primers.Keywords
This publication has 12 references indexed in Scilit:
- Initiation by Escherichia coli RNA‐polymerase: transformation of abortive to productive complexFEBS Letters, 1980
- E. coli RNA polymerase interacts homologously with two different promotersCell, 1980
- RNA polymerase unwinds an 11-base pair segment of a phage T7 promoterNature, 1979
- Primed abortive initiation of RNA synthesis by E. coli RNA polymerase on T7 DNA. Steady state kinetic studiesNucleic Acids Research, 1978
- A simple procedure for resolution of Escherichia coli RNA polymerase holoenzyme from core polymeraseArchives of Biochemistry and Biophysics, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- A rellable mapping method for sequence determination of oligodeoxyribonucleotides by mobility shift analysisAnalytical Biochemistry, 1976
- Bacteriophage T7 early promoters: Nucleotide sequences of two RNA polymerase binding sitesJournal of Molecular Biology, 1975
- Interaction of RNA Polymerase from Escherichia coli with DNAEuropean Journal of Biochemistry, 1975
- RNA Initiation with Dinucleoside Monophosphates during Transcription of Bacteriophage T4 DNA with RNA Polymerase of Escherichia coliProceedings of the National Academy of Sciences, 1973