Effect of metabolic inhibitors on the response of the toad bladder to vasopressin

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Abstract
This study was designed to explore the relationship between metabolism and the response to hormone. Iodoacetic acid (IAA), 10-4 [image], dinitrophenol (DNP), 10-3 [image], anaerobiosis (N2 acide, 10-3 [image], and fluoro-acetate (FAc), 10-2 [image], caused a fall in short-circuit current (SCC) and the SCC response to vasopressin. With the exception of FAc, each agent inhibited the permeability response to vasopressin as estimated by the flow of H2O along an osmotic gradient across the bladder. IAA, azide, and DNP inhibited the permeability response in a Ringer solution in which all sodium was replaced by choline. In general, the effect of inhibitors on the permeability response to cyclic 3[image],5[image]-AMP resembled the effect on the response to vasopressin. The data are interpreted as demonstrating a metabolic dependence of the SCC and permeability response of the toad bladder to vasopressin.