Production of Chimeric Mice from Cryopreserved Blastocysts.
- 1 January 1998
- journal article
- Published by Japanese Association for Laboratory Animal Science in Experimental Animals
- Vol. 47 (3) , 167-171
- https://doi.org/10.1538/expanim.47.167
Abstract
Mouse blastocysts derived from in vivo fertilization were cryopreserved by a simple vitrification method. Most of the cryopreserved blastocysts survived while demonstrating a normal morphology after warming (88.1%). The rate of cryopreserved blastocysts which developed to term as normal young was 56.8%, and chimeric mice were also produced from cryopreserved blastocysts injected with ES cells. As a result, 3.4% of the injected embryos developed to term as germ line chimeric mice.Keywords
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