Characterization of aPorphyromonas gingivalisgeneprtKthat encodes a lysine-specific cysteine proteinase and three sequence-related adhesins
- 1 April 1999
- journal article
- Published by Wiley in Oral Microbiology and Immunology
- Vol. 14 (2) , 92-97
- https://doi.org/10.1034/j.1399-302x.1999.140203.x
Abstract
Porphyromonas gingivalis extracellular arginine- and lysine-specific proteinases have been implicated as major virulence factors in the development of adult periodontitis. We have previously purified a 48-kDa lysine-specific cysteine proteinase, designated PrtK48, from a P. gingivalis W50 cell-associated multiprotein complex. PrtK48 was non-covalently associated with three sequence-related adhesins designated PrtK39, PrtK15 and PrtK44 in the multiprotein complex. In this study we cloned and characterized the gene, designated prtK, that encodes a polyprotein that is post-translationally processed to yield the Lys-specific proteinase PrtK48 and the three sequence-related adhesins PrtK39, PrtK15 and PrtK44.Keywords
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