ANTIIDIOTYPES TO ANTI-LOLP-I (RYE) ANTIBODIES IN ALLERGIC AND NONALLERGIC INDIVIDUALS - INFLUENCE OF IMMUNOTHERAPY

  • 1 October 1986
    • journal article
    • research article
    • Vol. 66  (1) , 231-240
Abstract
Anti-idiotypes (aId) reacting with anti-Lol I (Lolp I; Rye I) antibodies were detected by their ability to bind to radioiodinated F(ab'')2 anti-Lol I. Sera were tested after removal of anti-LolI and anti-heavy and light chain activity by adsorption on LolI-Sepharose 4B and normal human serum Sepharose 4B. The binding of aId to Id was inhibited by affinity purified anti-Lol I but not by certain unrelated immunoglobulins; in some sera this binding was also inhibited by LolI. The levels of aId were measured in serial bleedings collected over a 1 year period from LolI-sensitive patients, allergic donors not sensitive to LolI and non-allergic persons. In LolI-allergic patients the levels of aId were significantly influenced by seasonal exposure to pollen and by immunotherapy with extracts of grass pollen. Moreover, in 12 out of 16 cases, there was also a significant inverse relationship between changes in serum levels of aId and of IgG or IgE anti-LolI. Most interestingly, aId were also detected in non-allergic individuals; in this case, the levels of aId were not influenced by the pollen season. The data suggest that Id-aId interactions may play a role in the regulation of anti-LolI antibody production.

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