HEAT-RESISTANCE OF THE TUMORIGENIC NUCLEIC ACID OF SHOPE PAPILLOMATOSIS

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Abstract
Shope papilloma virus preparations which had been completely inactivated by heat at 701 yielded tumorigenic nucleic acid extracts by the phenolic deproteinization procedure. The tumor inducing capacity of the nucleic acid preparations obtained from papillomatous tissue of cottontail rabbits survived treatment at 100[degree] C for 30 minutes. It was suggested that since the melting point (Tm) of the extracted DNA was 90[degree]C any activity of the 100C treated DNA may be due to renaturation of the molecule. The possibility that single-stranded DNA is able to initiate neoplastic growths was also considered.