THEIN VITROREACTION OF CELLS TO ADRENAL CORTICAL STEROIDS WITH SPECIAL REFERENCE TO LYMPHOCYTES1

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Abstract
The reaction of lymphocytes to adrenal cortical steroids in vitro has been studied recently with contradictory results. Robertson (1948) and Delaunay, Delaunay and Lebrun (1949) reported no effects of adrenal cortical compounds on lymphocytes, while Schrek (1949) and Hechter and Johnson (1949) described a slight but definite toxic action. The work to be reported is a study of the action in vitro of adrenal cortical extracts, compound A, compound E and desoxycorticosterone upon lymphocytes, marrow and testicular cells, white cells of the peripheral blood and peritoneal washings of rabbits which consist mostly of polymorphonuclear neutrophiles (pseudoeosinophiles). method Schrek’s (1947) method of unstained cell counts, with some modifications, was employed. In brief, the technique consisted of making a suspension of cells in homologous serum or plasma, or in physiological salt media. A single animal provided both the serum or plasma and the cells,