Radioautographs in Which the Tissue is Mounted Directly on the Photographic Plate

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Abstract
Radioautographs of tissue containing radioactive I, for example, may be made by mounting the section directly on the photographic plate. This is done in the dark room, in front of a safe-light, by floating the paraffin sections on to the plate in water. After the prepn. has dried, it is stored in the dark for exposure to the radiation. The paraffin is removed with xylol and the plate (tissue still in place) is developed, fixed, washed, and dried. The tissue prepn. is then stained, dehydrated, cleared, and mounted in balsam. The final prepn. permits localization of the radio-active material under even high magnification as the autograph is automatically in place directly underneath the tissue. The technique has been employed in the study of radioactive I in the thyroid under exptl. conditions, in thyroid adenomas and carcinomas.