Laccase as a New Enzymatic Label for Enzyme Immunoassay
- 1 December 1994
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 27 (15) , 2997-3012
- https://doi.org/10.1080/00032719408000307
Abstract
A new immunochemical reagent is suggested containing as an enzyme marker laccase obtained from the cultural liquid of basidial fungi Coriolus hirsutus. The feasibility of immuno-laccase conjugates in different versions of immunoanalysis (sandwich, competitive and indirect enzyme immunoassay) was demonstrated. The assay based on antibodvlaccase conjugates is simpler than that employing antibody-peroxidase conjugates, since in the former case air oxygen is used as the second substrate of the enzymatic reaction.Keywords
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