STUDIES OF THE FINE STRUCTURE OF MICROORGANISMS II.Clostridium sporogenes

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Abstract
The sporulation process and the structure of the spores produced by synchronized cells of C. sporogenes were studied using stained preparations for light microscope observations and ultrathin sections prepared by an improved technique for parallel observation with the electron microscope. In the mature spore, an exosporium, two outer membranes and one inner membrane were observed. The outer membranes appeared first and enclosed a portion of cytoplasm containing an area of low electron density. The exosporium was formed next and the inner coat appeared last. With the formation of the inner coat, a change in spore texture occurred, and it was at this point that the spore lost affinity for basic dyes. In this organism, the cytoplasmic membrane of the sporangium did not shrink down to form a spore coat. Differences in the mode of spore formation between C. sporogenes and species in the genus Bacillus were discussed.

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