Stimulation of production of prostaglandin E in gingival cells exposed to products of human blood mononuclear cells

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Abstract
Supernatant media from cultures of unstimulated human peripheral blood mononuclear cells contained one or more factors that increased by several hundred-fold the production of prostaglandin E by fibroblast-like cells derived from inflamed and normal human gingival tissue. This stimulation occurred in a dose-dependent manner and was completely inhibited by 14 .mu.M-indomethacin. Responsiveness to the factor declined as the age of the cell culture increased. An increase in prostaglandin E production was first observed after a 2 h exposure to the mononuclear cell factor(s) and could be prevented by cycloheximide. Brief exposure (0.5 and 1-0 h) to mononuclear cell factor did not increase prostaglandin E production by the cells in a subsequent 72 h incubation in the absence of mononuclear cell factor. Addition of arachidonate (10 .mu.M and 15 .mu.M) further enhanced stimulation of prostaglandin E production in response to mononuclear cell factor. The stimulatory activity was resistant to digestion by trypsin, but was heat-labile, so that only 17% remained after treatment at 56.degree. C for 30 min.