Ultrastructural imaging of freeze-fractured plant cells in the scanning electron microscope
- 1 July 1992
- journal article
- research article
- Published by Wiley in Microscopy Research and Technique
- Vol. 22 (2) , 160-169
- https://doi.org/10.1002/jemt.1070220205
Abstract
The application of the freeze-fracture and cytoplasmic maceration technique in ultrastructural studies of plant cells is described. A major advantage of the technique is, that by extracting mobile cytoplasmic components from the freeze-fractured cells, surface relief is introduced and three-dimensional information is obtainable. The details of specimen preparation are described and the results obtained are reviewed. The use of chitosan embedding for very small or fragile specimens is described.Keywords
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