Epidermal growth factor (EGF), tumor promoter 12‐O‐tetradecanoylphorbol 13‐acetate (TPA) and calcium ionophore A23187 increase cytoplasmic free calcium and stimulate arachidonic acid release and PGE2/6‐keto PGF1α production in cultured porcine thyroid cells
- 10 December 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 225 (1-2) , 43-47
- https://doi.org/10.1016/0014-5793(87)81128-6
Abstract
Epidermal growth factor (EGF), 12-O-tetradecanoylphorbol 13-acetate (TPA) and calcium ionophore A23187 increase cytoplasmic free calcium ([Ca2+]i) and stimulate arachidonic acid release and production of PGE2 and 6-keto PGF1α an end metabolite of PGI2, in cultured porcine thyroid cells. Addition of EGF, TPA or A23187 to the cells loaded with fura-2, a fluorescent Ca2+ indicator, causes an immediate increase in [Ca2+]i which is the earliest event after mitogen stimulation. This [Ca2+]i response occurs immediately, reaching a maximum within several seconds. EGF, TPA and A23187 stimulate arachidonic acid release and PGE2 and 6-keto PGF1α production; the maximum effects are obtained after 2–4 h incubation. EGF, TPA and A23187 increase [Ca2+]i and then stimulate arachidonic acid release and PG productionKeywords
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