A pH stat for carbon dioxide incubator control.

Publisher Website
Google Scholar
Abstract
Commonly used methods of controlling the pH of bicarbonate-buffered tissue cultures within an incubator are inaccurate and extravagant in CO2. Moreover, technical problems which may result in the loss of cultures are common. The instrument described was designed to measure the pH of a solution containing the same concentration of bicarbonate as the medium in which the cells are being grown, and to use these data to control the amount of CO2 admitted to the incubator. Hence the pH of the culture will follow that of the measuring system. Observations are presented which demonstrate superior accuracy of control of pH within the culture medium, and much lower CO2 consumption than that of other methods. The system described provides a high degree of control over the pH of tissue cultures within an incubator. Various cell types were cultured over a 3-yr period, including mouse bone marrow cells, Chinese hamster lung fibroblasts and human lymphocytes. No technical faults occurred. The system is inexpensive to produce and can be fitted to any CO2 incubator. The system fails in the safer condition, that is, when the CO2 supply is off.

This publication has 2 references indexed in Scilit: