HIGHLY INCREASED PRODUCTION OF BONE MARROW-DERIVED BLOOD-CELLS BY ADMINISTRATION OF HOMOLOGOUS INTERLEUKIN-3 TO RHESUS-MONKEYS
- 1 December 1990
- journal article
- research article
- Vol. 76 (11) , 2235-2241
Abstract
Recombinant rhesus monkey interleukin-3 (IL-3) was administered to normal rhesis monkeys in graded doses ranging from 3 to 30 .mu.g/kg/d subcutaneously for 30 consecutive days or given as a continuous intravenous infusion at a dose of 30 .mu.g/kg/d for 16 days. After a lag phase of about 1 week, a highly increased, dose-dependent production of bone marrow-derived blood cells was observed, preceded by amplification of bone marrow hematopoietic progenitor cells. Simultaneously, peripheral blood progenitor cells rose. The increases included basophilic, eosinophilic and neutrophilic granulocytes, monocytes, and the erythrocyte and platelet lineages. Characeristically, a T-lymphocyte response was absent. It is concluded that IL-3 in vivo stimulates blood cell production from an immature, multipotent progenitor cell.This publication has 21 references indexed in Scilit:
- Stimulation of hematopoiesis in vivo by recombinant bacterial murine interleukin 3.Proceedings of the National Academy of Sciences, 1986
- A cloned MCGF cDNA encodes a multilineage hematopoietic growth factor: multiple activities of interleukin 3.The Journal of Immunology, 1985
- Interleukin-3 supports growth of mouse pre-B-cell clones in vitroNature, 1984
- Detection of murine bone marrow granulocyte/macrophage progenitor cells (GM‐CFU) in serum‐free cultures stimulated with purified M‐CSF or GM‐CSFThe International Journal of Cell Cloning, 1984
- Molecular cloning of cDNA for murine interleukin-3Nature, 1984
- Biologic properties of homogeneous interleukin 3. I. Demonstration of WEHI-3 growth factor activity, mast cell growth factor activity, p cell-stimulating factor activity, colony-stimulating factor activity, and histamine-producing cell-stimulating factor activity.The Journal of Immunology, 1983
- Procedures for the purification of interleukin 3 to homogeneity.The Journal of Immunology, 1982
- Phenotypic characteristics of cell lines requiring interleukin 3 for growth.The Journal of Immunology, 1982
- Antibodies to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man.The Journal of Experimental Medicine, 1981
- Thymus-dependent membrane antigens in man: Inhibition of cell-mediated lympholysis by monoclonal antibodies to T H 2 antigenProceedings of the National Academy of Sciences, 1981