The structure and nucleotide sequence of the 5' end of the human c-myc oncogene.

Publisher Website
Google Scholar
Abstract
The structure and nucleotide sequence of the 5'' end of the human c-myc oncogene was established using a cloned genomic fragment isolated from a fetal liver library (clone .lambda.MC41) and cloned c[complementary]DNA from the human leukemic cell line K562. The human c-myc oncogene consists of 3 exons and 2 introns. Primer extension of the human c-myc mRNA of 3 different cell lines and S1 nuclease protection experiments served to establish the position of 2 transcription initiation sites. The splicing site of the 1st exon-intron boundary was determined by comparative analysis of the sequences of the genomic and cDNA clones. The 1st exon contains termination codons in all 3 reading frames and no translation initiation signals, confirming a previous observation that the c-myc mRNA has a long 5'' noncoding sequence. This 1st exon also was found to be utilized in the formation of c-myc mRNA in a variety of human cell lines.