Improved detection of HLA-CWS and CW7 by two colour fluorescence technique
- 11 December 2008
- journal article
- research article
- Published by Wiley in Tissue Antigens
- Vol. 22 (5) , 372-378
- https://doi.org/10.1111/j.1399-0039.1983.tb02267.x
Abstract
Reactivity of 2 Cw5 and 3 Cw7 antisera was compared in the standard NIH [National Institutes of Health] and the 2 color fluorescence (TCF) techniques. Contrary to the NIH, the TCF method yielded strong positive reactions which enabled clear assignment of the Cw5 and Cw7 specificities. The TCF technique combines 3 factors that increase the sensitivity of serum reactions: typing on B lymphocytes; prolonged incubation; and staining of the lysed cells with ethidium bromide.This publication has 4 references indexed in Scilit:
- HLA Antibodies Cytotoxic only for B LymphocytesTissue Antigens, 2008
- Automated reading of HLA-A,B,C typing and screeningHuman Immunology, 1982
- Loss of HLA‐C locus specificities from stored lymphocytes1 and de novo synthesis following incubation at 37°CTissue Antigens, 1981
- Simultaneous detection of two cell populations by two-colour fluorescence and application to the recognition of B-cell determinantsNature, 1976