Adenylate energy charge in Acholeplasma laidlawii

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Abstract
ATP, ADP and AMP were produced by A. laidlawii B-PG9 growing in modified Edward medium. The adenylate energy charge was calculated to be 0.84 .+-. 0.07 and ranged from 0.91-0.78 during exponential growth (12-24 h). During exponential growth, A. laidlawii contained, at 17.5 h, 2.3 .times. 10-17 mol ATP per colony-forming unit and, at 16 h, 27.3 nmol ATP/mg (dry wt). The medium supported a doubling time of 0.95 h. The molar growth yields (Yglucose = g [dry wt]/mol glucose used) were 40.2 .+-. 3.4 (16 h) and 57.1 .+-. 9.7 (20 h) during midexponential growth. A maximum yield of 8.3 .times. 109 colony-forming units was reached at 24 h, when 56% of the initial concentration of glucose had been used. At 40 h, during the stationary phase, 14.95 .+-. 3.75 .mu.mol glucose/ml medium had been used. At this time, the culture fluids contained 21.86 .+-. 0.37 .mu.mol lactate/ml and 3.14 .+-. 0.13 .mu.mol pyruvate/ml.

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