Two new restriction endonucleases from Proteus vulgaris
Open Access
- 25 September 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (18) , 4525-4536
- https://doi.org/10.1093/nar/9.18.4525
Abstract
Two novel sequence-specific endonucleases have been isolated from Proteus vulqaris, ATCC 13315. PvuI recognizes the sequence: 5′ C G A T↓C G 3′ 3′ G C↑T A G C 5′ and PvuII recognizes the sequence: 5′ C A G↓C T G 3′ 3′ G T C↑G A C 5′ and cleave as indicated by the arrow (+). PvuI is an isoschizomer of XorII, RshI, and XniI. No enzyme with the specificity of PvuII has been described previously.Keywords
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