FACTORS NECESSARY FOR MAXIMUM GROWTH OF CLOSTRIDIUM BIFERMENTANS

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Abstract
The maximum growth of C. bifermentans in a vitamin-free casein hydrolysate medium was found to be dependent on added biotin, nicotinamide, pantothenate, and pyridoxamine. Some strain variation was found with regard to biotin and pyridoxamine. Nicotinic acid could be substituted for nicotinamine; pyridoxal could be substituted for pyridoxamine. Growth of C. bifermentans in an amino-acid medium was poor unless the medium was fortified by the addition of protein degradation products. Treatment of trypsin-digested casein with Chloramine-T did not affect the growth-promoting properties. Apparently some complex peptide was involved.
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