Clonal Variation In Proliferation Rate of Cultures of Gpk Cells

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Abstract
Pedigrees of 26 clones of a line of keratocytes derived from guinea pig ear epidermis (GPK cells) were analyzed from time-lapse film. The mean interdivision time (IDT) for the culture was 1143 .+-. 215 (SD) min. The mean generation rates (mean reciprocal interdivision times) of clones varied over a range of 3.93-10.2 .times. 10-4/min and the SD of the clonal mean generation rates was 16.8% of the average value. Transient intraclonal variations in IDT due to mitoses in a plane perpendicular to the substratum were observed. The data were also analyzed on the basis of cell location in 16 equal zones (quadrats) of the filmed area. The mean generation rate of quadrats was 8.73 .times. 10-4/min (SD = 4.9%). The spatial distribution showed some clustering of cells. The mean local density of the clones (2.25 .+-. 0.62 cells/10-4 cm2) was significantly higher than the quadrat density (1.76 .+-. 0.8 cells/10-4 cm2). There was no significant correlation between clonal density and mean generation rates; for quadrats a significant negative correlation was found (P = 2.7%). Cell lineage evidently is the major determinant of the proliferation rate of subconfluent cultures.