A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries
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Open Access
- 4 January 2011
- journal article
- method
- Published by Springer Nature in Genome Biology
- Vol. 12 (1) , R1
- https://doi.org/10.1186/gb-2011-12-1-r1
Abstract
Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.Keywords
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