TLA-2, a Novel Ambler Class A Expanded-Spectrum β-Lactamase

Abstract
β-Lactamase TLA-2 is encoded by a 47-kb plasmid isolated from an unidentified bacterial strain from a wastewater treatment plant. TLA-2 is an Ambler class A β-lactamase that shares 52% amino acid identity with CGA-1 from Chryseobacterium gleum and 51% with TLA-1 from Escherichia coli . The enzyme hydrolyzes mostly cephalosporins.

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