The Measurement of Bacterial Viabilities by Slide Culture

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Abstract
A procedure is described for measuring the viabilities (% viable/total organisms) of populations of Aerobacter aerogenes by short-term incubation on agar films followed by differential counting under the microscope. The only special apparatus needed is a supply of metal annuli. The procedure estimates viability with 95% fiducial limits of [plus or minus] 9%; it appears applicable to other aerobic bacteria, to yeasts and to spore suspensions.

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