A spectrofluorometric investigation, using 1-anilino-naphthalene-8-sulphonate, of the interaction between washed bovine spermatozoa and seminal plasma or egg-yolk lipoprotein

Publisher Website
Google Scholar
Abstract
The dye 1-anilino-naphthalene-8-sulfonate (ANS) bound at 94.5 .+-. 2.7 pmol per 106 spermatozoa (Kd = 1.83 .+-. 0.13 .times. 10-5 M) and 7.30 .+-. 0.17 pmol per mg seminal plasma protein (Kd = 2.19 .+-. 0.06 .times. 10-5 M). Equilibration of excess ANS with a mixture of washed spermatozoa and seminal plasma resulted in a significant increase in fluorescence over that calculated by summation of the individual levels (P < 0.05). A less pronounced but significant increase was seen when an egg-yolk lipoprotein, previously shown to have cryoprotective properties [when used in the storage of sperm], was similarly added to washed spermatozoa (P < 0.05). This increase in fluorescence was not reduced by washing spermatozoa-lipoprotein mixtures, suggesting that an interaction between the lipoprotein and the cell membrane occurred.