HPA‐1 typing by PCR amplification with sequence‐specific primers (PCR‐SSP): a rapid and simple technique
- 1 September 1993
- journal article
- Published by Wiley in British Journal of Haematology
- Vol. 85 (1) , 227-229
- https://doi.org/10.1111/j.1365-2141.1993.tb08680.x
Abstract
Summary. A DNA-based method was developed to genotype donors for the human platelet antigens HPA-la and -1b. Sequence-specific primers (SSP) were used in the polymerase chain reaction (PCR) which allowed the HPA-la/la. -lb/lb and -la/lb genotypes to be determined by PCR alone, no second analytical stage was required. 10 donors were tested by PCR-SSP and the results were concordant with serological phenotyping and independent DNA analysis.Keywords
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