Unrestricted recognition of class I neodeterminants generated by exon shuffling

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Abstract
We and others have previously reported that homologous exchange of ä1 or α2domains between different alleles led to loss of most determinants recognized by cytotoxic T lymphocytes (CTL) raised against the parental H‐2 class I antigens. Here we demonstrate that exchange of α1 or α2 domains between the Kk and Kd allele results in the formation of neodeterminants against which allospecific CTL can be generated in responder mice of various related and unrelated H‐2 haplotypes. In fact, only CTL against the neodeterminants on the hybrid molecules were found and none against the parental Kk and Kd determinants. In addition our data show that recognition of the neodeterminants is H‐2 unrestricted. These findings suggest that in the hybrid molecules the basic structure of a major histocompatibility complex molecule has been preserved although most parental allodeterminants on the α1 and α2 domains have been modified.

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