Low Temperature Sulfation of Tissues and the Demonstration Of Metachromasy
- 1 January 1957
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 32 (5) , 245-247
- https://doi.org/10.3109/10520295709111630
Abstract
Sulfation of tissue sections which contained nonmetachromatic polysaccharides was done in an equivolume mixture of concentrated sulfuric acid and glacial acetic acid at 0°C. The sections were washed successively in glacial acetic acid and water. Aqueous solutions of toluidine blue, 1.5 × 10-4 M, were used for staining. The procedure produced a metachromatic substrate in sites previously nonmetachromatic. Normal metachromasy was unaffected. There was no loss, damage or distortion of the tissue sections.Keywords
This publication has 3 references indexed in Scilit:
- METACHROMASIA; CHEMICAL THEORY AND HISTOCHEMICAL USEJournal of Histochemistry & Cytochemistry, 1956
- SULPHATION TECHNIQUES IN HISTOCHEMISTRY WITH SPECIAL REFERENCE TO METACHROMASIAJournal of Histochemistry & Cytochemistry, 1954
- Metachromasy of Toluidine Blue Induced by Nucleic Acids1Journal of the American Chemical Society, 1952